Phenol Red Indicator Solution
Phenol Red Indicator Solution is used as a pH indicator. The sodium salt of phenol red is used widely in culture media to identify changes from neutral to acidic pH values.
Code: RXSOL-60-6024-100
Other Equivalent Brand
Short Description:
Phenol Red Indicator Solution is used as a pH indicator. The sodium salt of phenol red is used widely in culture media to identify changes from neutral to acidic pH values
Phenol Red Indicator Solution
Application:
It is typically used in cell culture media at 11 mg/L. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH of 8.2 and above.Phenol red in tissue culture m.
It is typically used in cell culture media at 11 mg/L. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH of 8.2 and above.Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. Lipophilic impurities, not the phenol red dye itself, account for the estrogenic activity. 95-99% of these impurities can be removed from the sodium salt of phenol red with a reduction in estrogen-like activity.
Using Procedure:
Preparation Instructions This product is a sterle filtered 0.5% solution of tissue culture grade phenol red in Dulbecco,s phosphate buffered saline without magnesium and calcium. An indicator solution c.
Preparation Instructions
This product is a sterle filtered 0.5% solution of tissue culture grade phenol red in Dulbecco,s phosphate buffered saline without magnesium and calcium.
An indicator solution can be formed by dissolving 0.1 g of phenol red in 14.20 ml of 0.02 N NaOH and diluted to 250 ml with deionized water. Phenol red can be used to measure hydrogen peroxide in cultured macrophages in multiwell plates. Assays used 2 units of peroxidase/100 ml volume with 0.5 mM phenol red, pH 7 at 37 °C. The reaction was stopped and color developed by adding 10 ml of 1 N NaOH and reading the absorption of the oxidized phenol red at 600-610 nm. Calculations were accomplished by comparison to a standard curve of hydrogen peroxide.
Note:
Precautions and Disclaimer : For Laboratory Use Only. Not for drug, household or other uses. Storage/Stability Phenol red when added as a component to tissue culture media, can be autoclaved.
Technical Specifications:
Physical State |
: Liquid |
Color |
: Red to orange |
Odor |
: Odorless |
pH |
: 7-8 |
Specific Gravity |
: 1.0 |
Solubility in water |
: Soluble
|
Remarks:
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Packing Size: 1.00 Bottle
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