MUSCAT CHEMICAL TAG::
Staining according to Neisser is a test for the presence of polyphosphates stored in the cells (= storage materials). This method is an indispensable aid to the identification of certain strains of filamentous bacteria.
The performance of the Gram Stain on any sample requires four basic steps that include applying a primary stain (crystal violet) to a heat-fixed smear, followed by the addition of a mordant (Gram's Iodine), rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone and lastly, counterstaining
Chrysoidine solution. Density (D) ~1 g/cm³. Boiling point (bp) ~100 °C Flash point (flp) >60 °C WGK 1. Solution for diphtheria staining acc. to Neisser.