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The uranyl acetate (UA), which enhances the contrast by interaction with lipids and proteins, forms a yellow, needle-like crystal precipitate if not used in the right concentration and if redundant UA is not removed from the section.
Uranyl acetate is extensively used as a negative stain in electron microscopy. Most procedures in electron microscopy for biology require the use of uranyl acetate. ... Uranyl acetate dihydrate has been used as a starting reagent in experimental inorganic chemistry.
Uranyl acetate produces negative stain contrast in TEM micrographs due to its exclusion from the dense ordered peptide structures, providing dark highlights along the fiber edges.
