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Van Gieson's trichrome is used for the differentiation between collagen and smooth muscle in tumors and to demonstrate the increase in the amount of collagen in these pathologies.
Van Gieson Stain is used to differentiate between collagen and smooth muscle in tumors and to demonstrate the increase of collagen in diseases. This method combines two or more anionic dyes and rely on differential binding by tissue components.
Use 4 ml of distilled water to dissolve potassium iodide. And then add iodine. Once iodine is dissolved, dilute this solution by adding 96 ml of distilled water. This solution may be prepared fresh as needed or made in larger quantities and stored in brown bottle in the dark at room temperature.
