Product Application
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In aqueous solution, bromocresol green will ionize to give the monoanionic form (yellow), that further deprotonates at higher
pH to give the dianionic form (blue), which is stabilized by resonance The acid dissociation constant (pKa) of this reaction
BROMOCRESOL GREEN INDICATOR SOLUTION used as a pH indicator and as a tracking dye for DNA agarose gel electrophoresis. It can be used in its free acid form (light brown solid), or as a sodium salt (dark green solid). Intended Use Bromo Cresol Green Indicator is recommended as a pH indicator in growth medium for microorganisms.
Between pH values of 3.8 and 5.4 it appears green. The dye is only sparingly soluble in water, so it may be dissolved in ethanol or certain organic solvents
